Hepatitis C virus core protein-induced miR-93-5p up-regulation inhibits interferon signaling pathway by targeting IFNAR1

Figure 5 The miR-93-5p-IFNAR1 axis regulates the interferon signaling pathway. A: Western blot shows the protein expression of IFNAR1 in Huh7 cells transfected with NC (200 ng/mL), siRNA (200 ng/mL), vector (200 ng/mL), or IFNAR1 (200 ng/mL). This experiment was repeated twice; B and C: Western blot shows the phosphorylation level of STAT1 and the protein expression of STAT1 in Huh7 cells transfected with NC (200 ng/mL), siRNA (200 ng/mL), vector (200 ng/mL), or IFNAR1 (200 ng/mL). This experiment was repeated twice; D and E: Western blot shows the phosphorylation level of STAT1 and the protein expression of STAT1 in Huh7 cells transfected with ago-NC (200 nmol/L), miR-93-5p agomir (200 nmol/L), antago-NC (200 nmol/L), or miR-93-5p antagomir (200 nmol/L). This experiment was repeated twice; F and G: Western blot shows the phosphorylation level of STAT1 and the protein expression of STAT1 and IFNAR1 in Huh7 cells transfected with ago-NC (200 nmol/L), miR-93-5p agomir (200 nmol/L), or miR-93-5p agomir (200 nmol/L) plus IFNAR1 (200 ng/mL). IFNAR1: Interferon receptor 1.