Characterization of biofilms in biliary stents and potential factors involved in occlusion

Table 1 Primer sequences used for polymerase chain reaction

Sr. No	Organism	Primer sequences
1	Pseudomonas	F: 5'-GACGGGTGAGTAATGCCTA- 3'
		R: 5'-CACTGGTGTTCCTTCCTATA -3'
2	Staphylococcus	F: 5'- AAC TCT GTT ATT AGG GAA GAA CA- 3'
		R: 5'- CCA CCT TCC TCC GGT TTG TCA CC-3'
3	E. coli	F: 5'-GAAGCTTGCTTCTTTGCT- 3'
		R: 5'-GAGCCCGGGGATTTCACAT- 3'
4	Enterococcus	F: 5'- GTTTATGCCGCATGGCATAAGAG -3'
		R: 5'-CCGTCAGGGGACGTTCAG -3'
5	Citrobacter	F: 5′-TCAGATTTGAACGCTGGCGGCA -3′
		R: 5′-CGTATTACCGCGGCTGCTGCCAC -3′
6	Proteus	F: 5'-AGA GTT TGA TCC TGG CTC AG-3'
		R: 5'-AAG GAG GTG ATC CAG CC-3'
7	Klebsiella	F: 5'-AGA GTT TGA TCC TGG CTC AG-3'
		R: 5'-AAG GAG GTG ATC CAG CC-3'
8	Clostridium	F: 5'-TGG CTC AGA TTG AAC GCT GGC GGC -3'
		R: 5'-TAC CTT GTT ACG ACT TCA CCA CA-3'
9	Bacillus	F:5'- AGA GTT TGA TCC TGG CTC AG -3'
		R: 5'- AAG GAG GTG ATC CAG CCG CA-3'
10	Vibrio	F: 5'-AGA GTT TGA TCA TGG CTC AG -3'
		R:5'-GAA ATT CTA CCC CCC TCT ACA G-3'
11	Aeromonas	F: 5'-GCT GGT CTG AGA GGA TGA TC-3'
		R: 5'-CTT TAC GCC CAG TAA TTC CG-3'
12	Bacteroides	F: 5'- ATT CTA GAG TTT GAT CAT GGC TCA-3'
		R: 5'-ATG GTA CCG TGT GAC GGG CGG TGT GTA-3'
13	Enterobacter	F: 5'-AGTTTGATCCTGGCTCAG -3'
		R: 5'-TAC CTT GTT ACG ACT TCG TCC CA-3'
14	Streptococcus	F: 5'-TAA CCA GAA AGG GAC GGC TA-3'
		R: 5'-CAC TCT CCG CTT CTG CAC TC-3'
15	Serratia	F : 5'-GCGGTTTGTTAAGTCAGATG -3'
		R: 5'-CGAATTAAACCACATGCTCC-3'
16	Yersinia	F : 5′-AAT ACC GCA TAA CGT CTT CG-3′
		R: 5′-CTT CTT CTG CGA GTA ACG TC-3′