Proteomic profiling of fetal esophageal epithelium, esophageal cancer, and tumor-adjacent esophageal epithelium and immunohistochemical characterization of a representative differential protein, PRX6

Table 1 Identification of differentially expressed proteins in tumor-adjacent normal esophageal epithelial tissue, esophageal squamous cell carcinoma tissue, and fetal esophageal tissue

Spot	Protein name and theoretical pI and Mr	Observed pI and Mr	No. of matched peptide fragments	Sequence coverage	MOWSE score
2	USP30 6.87/45546	9.3/32	2	4	21
4	Thyroid hormone receptor interactor 4.56/11 8489	7.8/31	3	56	51
5	Cardiac muscle 5.34/22563	6.25/29	5	24	66
6	Chain A, crystal structure of a recombinant glutathione transferase 5.09/23202	6.2/24	5	43	85
7	Dermatopontin precursor 4.7/23989	5.3/21.5	5	29	82
10	Heat shock protein 27 7.83/22313	7.1/20.5	5	29	79
11	S100 calcium-binding protein A9 5.71/13234	6.3/15	5	40	74
12	G-gamma-hemoglobin 6.17/11033	7.5/16	3	45	65
13	MB protein 9.24/10863	8.4/15.5	3	32	65
14	Immunoglobulin heavy chain variable region 9.78/13026	8.6/14	3	34	70
15	PREDICTED: hypothetical protein 5.27/6706	8.1/12	2	44	52
16	Immunoglobulin heavy chain variable region 9.04/13053	7.1/11	2	26	49
17	Heat shock 27kDa protein 1 5.98/22768	9.2/16	6	33	80
18	hCG1659706 10.28/5287	9.5/21	2	64	37
19	Smooth muscle protein 8.56/22461	9.4/19	6	26	86
21	hCG1997574 9.07/29453	7.3/32	7	31	93
22	Heat shock 27 kDa protein 1 5.98/22768	6.8/32	6	35	84
23	Disabled homolog 1 (Drosophila) 9.40/22903	7.15/31	6	26	66
24	Phosphoglycerate mutase 1 (brain), 6.40/24669	7.3/31	8	40	117
25	Peroxiredoxin 6 6.00/25019	7.1/27.8	8	41	116
26	Chain A, human triosephosphate isomerase of new crystal form 6.50/26666	7.3/30	10	38	160