Down-regulation of miR-30a-3p/5p promotes esophageal squamous cell carcinoma cell proliferation by activating the Wnt signaling pathway

Figure 5 Down-regulation of miR-30a-3p/5p expression enhances the activity of Wnt signaling pathway. A: Respective KEGG pathway enrichment analyses of miR-30a-3p (top) and miR-30a-5p (bottom) target genes; B: Luciferase reporter assay suggested that down-regulation of miR-30a expression enhanced the activity of Wnt signaling pathway, while over-expression of miR-30a attenuated it; C: Western blot assay indicated that miR-30a negatively regulated the protein expression of downstream genes of the Wnt signaling pathway, including Cyclin D1, p27, and p21. α-tubulin was used as a loading control; D, E and F: qPCR assay revealed that miR-30a negatively regulated the mRNA expression of Cyclin D1, p27, and p21. Error bars represent the mean ± SD of three independent experiments; ^aP < 0.05.