Chitinase 3-like 1 secreted by peritumoral macrophages in esophageal squamous cell carcinoma is a favorable prognostic factor for survival

Figure 3 Expression level of chitinase 3-like 1 in monocyte-derived macrophages after stimulation of either supernatant of esophageal squamous cell carcinoma cell line Eca-109 or interleukin-6. A: Expression of CHI3L1 mRNA and protein in monocyte-derived macrophages (upper, mRNA; lower, protein) after stimulation of Eca-109 cell line supernatant was analyzed by real-time PCR and ELISA, respectively; B: Cytokine levels of Eca-109 supernatant were measured with BD CBA Th1/Th2/Th17 Cytokine Kit; C: Level of CHI3L1 mRNA (left) and protein (right) in monocyte-derived macrophages after stimulation of interleukin-6 (IL-6) was analyzed by real-time PCR and ELISA, respectively. Error bars represent standard deviations (SD) calculated from three parallel experiments. CHI3L1: Chitinase 3-like 1.