Glucagon-like peptide-2 modulates the nitrergic neurotransmission in strips from the mouse gastric fundus

Figure 2 Lack of effects of glucagon-like peptide-2 on the neurally-induced contractile responses in the presence of the nitric oxide synthesis inhibitor L-NNA. A: Typical tracing showing the electrical field stimulation (EFS)-induced contractile responses at different stimulation frequencies (left panel). Following the addition of L-NNA (200 µmol/L) to the bath medium, the amplitude of the EFS-induced excitatory responses is enhanced (middle panel). In the presence of L-NNA, glucagon-like peptide-2 (GLP-2, 20 nmol/L) no longer depresses the amplitude of the neurally-induced contractile responses (right panel); B: Bar chart of the effects of GLP-2 (20 nmol/L) on the mean amplitude of the EFS-induced contractile responses in the presence of L-NNA (200 µmol/L). Excitatory responses are expressed as percentage of the muscular contraction evoked by 2 μmol/L methacholine, taken as 100%. All values are mean ± SE of six preparations. ^aP < 0.05 vs the ctr ^bP < 0.05 and P > 0.05 vs the ctr and vs GLP-2, respectively (ANOVA and Newman-Keuls post-test).