Basic Study
Copyright ©The Author(s) 2017.
World J Gastroenterol. Jan 28, 2017; 23(4): 603-613
Published online Jan 28, 2017. doi: 10.3748/wjg.v23.i4.603
Figure 1
Figure 1 SDS-PAGE (10%) analysis for nickel agarose affinity chromatography purification of fusion protein. A new protein band with a molecular weight of approximately 55 kDa appeared in the 500 mmol/L imidazole elution fractions. M: Protein marker; 1: Sample; 2: Outflow; 3: 20 mmol/L Imidazole elution fractions; 4, 5: 50 mmol/L Imidazole elution fractions; 6, 7: 500 mmol/L Imidazole elution fractions.