Myo-inositol reduces β-catenin activation in colitis

Figure 3 pβ-cat and pAkt are reduced in colitis mice treated with myo-inositol. A: Representative images of pβ-cat and pAkt staining; B: Quantification of the number of pβ-cat- and pAkt-positive nuclei. Distribution of the percent of crypts containing 0-5 pβ-cat-positive nuclei. Values represent the mean ± SE, n = 4 mice in each group. ^aP < 0.05; C: Western blots of IEC showing levels of nuclear pβ-cat and pAkt in the 8 cycle model of DSS-induced colitis. Lanes were rearranged to be consistent with formatting. Densitometry was normalized to β-actin and presented as the fold change (mean ± SE). n = 2 control mice, 3 DSS-treated mice, and 3 DSS/myo-inositol-treated mice; D: TOPFlash reporter assay (shown in relative fluorescence units, RFU) for β-catenin activation in NCM460 cells treated with TNF or myo-inositol. n = 4 separate experiments.