Anti-oxidant and anti-inflammatory effects of hydrogen-rich water alleviate ethanol-induced fatty liver in mice

Figure 4 Anti-inflammatory effect of hydrogen-rich water on cytokines in chronic-binge ethanol-fed C57BL/6J mice. A and B: The levels of (A) TNF-α and (B) IL-6 in serum were measured as pro-inflammatory markers; C and D: In relative terms, (C) IL-10 and (D) IL-22 in serum were measured as anti-inflammatory markers. The values representare the mean ± SD from 8-10 mice for each group and at least three independent measurements. ^aP < 0.05 vs control group, ^cP < 0.05 vs EtOH group, and ^eP < 0.05 vs silymarin group. EtOH: Ethanol; HRW: Hydrogen-rich water; IL: Interleukin; Sily: Silymarin; TNF-α: Tumor necrosis factor-alpha.