Genetic association and epistatic interaction of the interleukin-10 signaling pathway in pediatric inflammatory bowel disease

Table 3 PCR-RFLP method for genotyping IL-10, IL10RA, IL10RB, STAT3 and HO1 genes

Gene	SNP ID	Variant	PCR amplification		RFLP
			Primers1	Product, bp	Restriction enzyme	Recognition site
IL-10	rs1800872	G>T	IL-2f: 5'-AACTTAGGCAGTCACCTTAGG-3'	149	ScaI	T yes; G No
			IL-2r: 5'-CATCCTGTGACCCCTCCAGT-3'
	rs3024498	T>C	IL-5f: 5'-GCTCCtTGGTTTCTCTTCCTA AG-3'	137	HpyCH4V	C yes; T No
			IL-5r: 5'-AGAAGCTTCCATTCCAAGCC TGA-3'
	rs3024496	A>G	IL-4f: 5'-GTATCAGAGGTAATAAATATTCcAT-3'	178	NlaIII	G yes; A No
			IL-4r: 5'-TAGAAGCATACATGACAATGAAG-3'
IL10RA	rs3135932	A>G	RA3f: 5'-CCCGCAAATGACACATATGgA-3'	172	MnI	G yes; A No
			RA3r: 5'-AGTTCCCAATGGCACACAAGG-3'
IL10RB	rs2834167	A>G	RB3f: 5'-GCCATAGAGGAGAACCAAGTG-3'	206	CarI	G yes; A No
			RB3r: 5'-GCTGTGAAAGTCAGGTTCTCTT-3'
STAT3	rs744166	A>G	ST2f: 5'-CAGGAGTGCCAACATTGAGAG-3'	106	AluI	A yes; G No
			ST2r: 5'-G TAATGTCTTGAGGGAATCGAG-3'
HO1	rs2071746	A>T	HO4f: 5'-TCAGCAGAGGATTCCAGCAGG-3'	110	BfaI	A yes; T No
			TG-3' HO4r: 5'-AGGCAGCGCTGCTCAGAGCAC-3'

¹Lowercase letter indicates a mismatched nucleotide.