Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice

Figure 5 IL-33-induced IL-13 expression was Fn14 dependent. A: IL-33 levels were measured from homogenate of the ileum after injection of 5-FU (n = 4 mice per time point). Open bar, WT mice; solid bar, Fn14 KO mice (n = 4 mice per time point). Data are shown as mean ± SD (^aP < 0.05); B: Fold increase of IL-33 mRNA in ECs and LPCs on day 5 after injection of 5-FU (n = 3 mice per experiment). Data are shown as mean ± SD; C: Sections were prepared from samples collected on days 0 (naïve), 5, and 8 after injection of 5-FU from WT and Fn14 KO mice and probed with anti-IL-33 antibody (green) (n = 3 mice per experimental condition). Red: Nuclear staining. Scale bar = 100 µm. Representative images are provided; D: LPCs from naïve WT or Fn14 KO ileum were stimulated with recombinant IL-33 in vitro and the number of IL-13-producing cells per mouse was measured using flow cytometry. Results of repeated experiments (n = 3) are summarized.