Relationship of serum polyunsaturated fatty acids with cytokines in colorectal cancer

Table 3 Multivariate analysis of the relationship between n-6, n-3 polyunsaturated fatty acid indicators and cytokines

	n-6 PUFA/n-3 PUFA1					C20:4 n-6/(C20:5 n-3 + C22:6 n-3)1
Quartiles limits	< 8.47	8.47-10.25	10.25-12.66	> 12.66	P value for trend	< 2.64	2.64-3.21	3.21-3.94	> 3.94	P value for trend
Median	7.49	9.41	11.64	14.08		2.16	2.95	3.56	4.79
IFN-γ (pg/mL)	0.45	0.46	0.30	0.41	0.240	0.27	0.55	0.55	0.30	0.157
IL-8 (pg/mL)	23.82	70.90	48.43	50.67	0.495	18.58	53.84	63.53	60.30	0.413
TNF-α (pg/mL)	7.01	10.53	6.62	7.45	0.600	6.81	9.20	7.98	7.36	0.094
MMP-2 (ng/mL)	5.80	14.95	11.55	11.37	0.549	4.12	11.48	14.13	11.37	0.980
MMP-9 (ng/mL)	9.88	11.81	11.27	12.24	0.734	9.73	10.30	12.56	12.26	0.495
	C20:4 n-6/C20:5 n-31					C20:4 n-6/(n-6 PUFA + n-3 PUFA)1
Quartiles limits	< 16.65	16.65-25.18	25.18-39.20	> 39.20	P value for trend	< 0.18	0.18-0.21	0.21-0.23	> 0.23	P value for trend
edian	12.66	19.67	30.67	48.51		0.16	0.20	0.22	0.25
IFN-γ(pg/mL)	0.29	0.46	0.46	0.30	0.906	0.41	0.27	0.55	0.46	0.769
IL-8 (pg/mL)	26.78	41.71	80.35	50.88	0.978	23.13	43.41	71.56	65.01	0.108
TNF-α (pg/mL)	6.63	8.01	8.47	8.30	0.334	6.29	8.15	8.70	8.40	0.432
MMP-2 (ng/mL)	8.99	10.84	12.81	13.77	0.977	9.10	11.35	12.24	12.81	0.200
MMP-9 (ng/mL)	9.73	11.32	12.59	12.14	0.058	10.85	10.52	12.57	11.90	0.350

¹The association of n-6 PUFA and cytokines were adjusted for potential confounding factors, including age, sex, smoke, alcohol drinking, body mass index, total cholesterol, low density lipoprotein cholesterol, high density lipoprotein cholesterol, triglycerides, total energy, protein, fat and carbohydrate intake. PUFA: Polyunsaturated fatty acids; IFN-γ: Interferon gamma; IL-8: Interleukin-8; TNF-α: Tumor necrosis factor-α; MMP-2: Matrix metalloproteinase-2.