Nicotinic cholinergic receptors in esophagus: Early alteration during carcinogenesis and prognostic value

Table 5 Association of CHRN expression fold-change and clinicopathological data of esophageal squamous cell carcinoma patients

Clinicopathologicaldata	CHRNA3		CHRNA5		CHRNA7		CHRNB4
	FC,	P value	FC,	P value	FC,	P value	FC,	P value
	median		median		median		median
	(min-max)		(min-max)		(min-max)		(min-max)
Age, yr	60		59.5		59.5		60
	(46-79)		(46-79)		(46-79)		(46-79)
> median	1.1	0.2775	2.166	0.1612	2.867	0.9451	1.984	0.1128
	(0.03136-14.69)		(0.3918-15.07)		(0.2630-20.53)		(0.1586-7.119)
≤ median	0.5844		3.193		1.267		0.7518
	(0.006324-10.62)		(0.8615-11.66)		(0.4813-23.10)		(0.1414-2.051)
Sex
Men	0.9777	0.1053	3.032	0.1719	2.867	0.7192	1.685	0.1053
	(0.006324-14.69)		(0.3918-15.07)		(0.4813-23.10)		(0.2261-7.119)
Women	0.1153		2.102		1.657		0.3153
	(0.03349-1.072 )		(0.5510-3.593)		(0.2630-20.53)		(0.1414-2.809)
Smoking
Never	NA	NA	NA	NA	NA	NA	NA	NA
Ever	0.6941		3.01		1.86		1.229
	(0.006324-14.69)		(0.3918 - 15.07)		(0.2630-23.10)		(0.1414-7.119)
Alcohol consumption
Never	NA	NA	NA	NA	NA	NA	NA	NA
Ever	0.5844		2.971		1.657		0.9571
	(0.006324-14.69)		(0.3918-15.07)		(0.2630-23.10)		(0.1414-7.119)
Tumor localization1
Upper third	0.1153	0.4079	1.589	0.0737	0.8255	0.1525	0.3964	0.3510
	(0.03136-14.69)		(0.3918- 6.284)		(0.2630-7.917)		(0.1414-7.119)
Middle third	0.8265		3.01		2.567		1.5
	(0.006324-10.62)		(0.8615-11.66)		(0.4813-23.10)		(0.2261-2.809)
Lower third	3.904		9.329		4.199		2.211
	(0.07381-7.799)		(3.414-15.07)		(0.7220-13.36)		(1.613-2.552)
Histological grade, differentiation
Well	NA	0.4902	NA	0.9767	NA	0.3661	NA	0.8610
Moderate	0.8039		3.031		1.454		0.9571
	(0.03136-14.69)		(0.3918-15.07)		(0.2630-23.10)		(0.1414-7.119)
Poor	0.2908		2.6		2.514		1.5
	(0.006324-1.626)		(1.826-4.993)		(1.057-18.57)		(0.5035-2.532)
Tumor stage
1 + 2	0.5728	0.9599	2.505	0.778	1.454	0.7780	1.832	0.4457
	(0.03349-10.62)		(1-15.07)		(0.4813-20.53)		(0.1414-2.809)
3 + 4	0.8039		3.01		3.167		0.9571
	(0.006324-7.799)		(0.4633-6.566)		(0.6082-23.10)		(0.2261-2.532)

¹The analyses were performed considering only the upper and middle thirds because the number of individuals with ESCC in the lower third was too small. Statistical analyses were performed using Mann-Whitney or Kruskal Wallis test and Dunn's post-test, as appropriate; no significant differences were observed. NA: Not applicable because the number of individuals was too small.