Nicotinic cholinergic receptors in esophagus: Early alteration during carcinogenesis and prognostic value

Table 1 Sequences of the oligonucleotides used in reverse transcription-quantitative PCR

Gene	Oligonucleotide sequences (5'-3')	Positive control	Ref.
CHRNA1	Forward: ACCAGGAGTCTAACAATGCG	Glioblastoma cell line, U251	Designed by the authors
	Reverse: ACAAGCATGAAGACTCCGAG
CHRNA3	Forward: AACCTGTGGCTCAAGCAAATCT	Lung cancer cell line, A549	[20]
	Reverse: CATGAACTCTGCCCCACCAT
CHRNA4	Forward: ACACAGACTTCTCGGTGAAG	ESCC cell line, TE1	Designed by the authors
	Reverse: CAGCAGGCAGACGATGATGA
CHRNA5	Forward: AGATGGAACCCTGATGACTATGGT	Lung cancer cell line, A549	[20]
	Reverse: AAACGTCCATCTGCATTATCAAAC
CHRNA7	Forward: GCTGCTCGTGGCTGAGATC	Lung cancer cell line, A549	[20]
	Reverse: TGGCGAAGTACTGGGCTATCA
CHRNA9	Forward: GATGGCCTAGACTCCATCAG	Glioblastoma cell line, U251	Designed by the authors
	Reverse: CTGAAGATTCATCATCAGCCTTG
CHRNA10	Forward: CTACTCCCTGCAGAGTGCCTG	Glioblastoma cell line, U251	Designed by the authors
	Reverse: TCTGGTCTGTGTCTGCCACAG
CHRNB4	Forward: TCACAGCTCATCTCCATCAAGCT	Lung cancer cell line, A549	[20]
	Reverse: CCTGTTTCAGCCAGACATTGGT
GAPDH	Forward: CAACAGCCTCAAGATCATCAGCAA	-	[22]
	Reverse: AGTGATGGCATGGACTGTGGTCAT

ESCC: Esophageal squamous cell carcinoma.