Retrospective Cohort Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Aug 21, 2016; 22(31): 7146-7156
Published online Aug 21, 2016. doi: 10.3748/wjg.v22.i31.7146
Table 1 Sequences of the oligonucleotides used in reverse transcription-quantitative PCR
GeneOligonucleotide sequences (5'-3')Positive controlRef.
CHRNA1Forward: ACCAGGAGTCTAACAATGCGGlioblastoma cell line, U251Designed by the authors
Reverse: ACAAGCATGAAGACTCCGAG
CHRNA3Forward: AACCTGTGGCTCAAGCAAATCTLung cancer cell line, A549[20]
Reverse: CATGAACTCTGCCCCACCAT
CHRNA4Forward: ACACAGACTTCTCGGTGAAGESCC cell line, TE1Designed by the authors
Reverse: CAGCAGGCAGACGATGATGA
CHRNA5Forward: AGATGGAACCCTGATGACTATGGTLung cancer cell line, A549[20]
Reverse: AAACGTCCATCTGCATTATCAAAC
CHRNA7Forward: GCTGCTCGTGGCTGAGATCLung cancer cell line, A549[20]
Reverse: TGGCGAAGTACTGGGCTATCA
CHRNA9Forward: GATGGCCTAGACTCCATCAGGlioblastoma cell line, U251Designed by the authors
Reverse: CTGAAGATTCATCATCAGCCTTG
CHRNA10Forward: CTACTCCCTGCAGAGTGCCTGGlioblastoma cell line, U251Designed by the authors
Reverse: TCTGGTCTGTGTCTGCCACAG
CHRNB4Forward: TCACAGCTCATCTCCATCAAGCTLung cancer cell line, A549[20]
Reverse: CCTGTTTCAGCCAGACATTGGT
GAPDHForward: CAACAGCCTCAAGATCATCAGCAA-[22]
Reverse: AGTGATGGCATGGACTGTGGTCAT