Immunohistochemistry panel segregates molecular types of hepatocellular carcinoma in Brazilian autopsy cases

Table 6 General correlation between expression scores for immunohistochemical markers and categories of histological grade for primary hepatocellular carcinoma

	Grade	Ki67	K19	p53	ERK1	BcatN	ERK2	Vim	CKD1	BcatM	mTOR	MET	EGFR	Group No.
Ki67	0.43a													Group 1
K19	0.37b	0.03
p53	0.36c	0.29d	0.08
ERK1	0.34c	0.39b	0.37b	0.17
BcatN	0.23	0.44a	0.13	0.24	0.18
ERK2	0.19	0.63a	0.22	-0.10	0.36c	0.24
Vim	0.14	0.11	0.51a	0.02	0.31c	0.08	0.14
CKD1	0.03	0.46a	-0.32c	-0.10	0.03	0.26d	0.29d	-0.19						Group 2
BcatM	-0.08	0.26d	-0.01	-0.23	0.17	-0.07	0.42a	0.01	0.25d
mTOR	-0.24d	-0.02	-0.20	-0.21	0.25d	-0.03	0.13	-0.13	0.17	0.44a
MET	-0.32c	0.16	-0.20	-0.35c	-0.01	0.22	0.39b	-0.18	0.38b	0.18	0.40b
EGFR	-0.34c	0.00	-0.14	-0.13	-0.04	-0.24	0.22	-0.07	0.13	0.20	0.11	0.23		Group 3
Casp3	-0.42a	-0.18	-0.25d	-0.46a	-0.19	-0.06	0.06	-0.27d	0.19	0.16	0.32b	0.41a	0.35c

^aP < 0.0005,

^bP≤ 0.001,

^cP < 0.01,

^dP < 0.05 by Spearman’s rho. Cases with positivity (group 1) and negativity (group 3) for immunohistochemical markers as associated with the histological grade and cell proliferation; Cases in group 2 showed intermediate features, between groups 1 and 3. BcatM: Membrane beta-catenin; BcatN: Nuclear beta-catenin; Casp3: Caspase 3; CKD1: Cyclin D1; EGFR: Epidermal growth factor receptor; HCC: Hepatocellular carcinoma; mTOR: Mechanistic target of rapamycin; Vim: Vimentin.