Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

Figure 3 TRPV4-mediated increases in cytosolic Ca²⁺ ([Ca²⁺]_i) in mouse primary gastric epithelial cells. A: [Ca²⁺]_i changes (340/380 ratio) in response to the TRPV4 specific agonist GSK1016790A (GSK, 100 nmol/L) in WT or TRPV4KO (V4KO) primary gastric epithelial cells (mean ± SEM). Ionomycin (iono, 5 μmol/L) was used as a positive control. Bars indicate the period of chemical application; B: GSK significantly increased [Ca²⁺_]i in WT cells (means ± SD; 1.03 ± 0.07, n = 20) compared to TRPV4KO cells (0.05 ± 0.01, n = 20) (^bP < 0.01 vs WT).