Tumor-specific expression of shVEGF and suicide gene as a novel strategy for esophageal cancer therapy

Figure 4 Effects of shVEGF-yCDglyTK/5-FC system on EC9706 cells. A: Cell viability of parental and stable EC9706 cells were determined with MTT assay at various time points after 5-FC treatment. The results shown are representative of three independent experiments; B: Representative images of Hoechst 33258-stained nuclei at magnification × 200. Apoptotic nuclei are condensed or fragmented; parental EC9706 cells (a); EC9706/null (b); EC9706/shVEGF (c); EC9706/yCDglyTK (d); EC9706/shVEGF-yCDglyTK (e); C: Representative dot plots of flow cytometry analysis. The numbers represent the percentage (%) of cells. Upper right quadrant, late apoptosis; lower right quadrant, early apoptosis; lower left, live cells. 5-FC: 5-fluorocytosine.