Altered tryptophan hydroxylase 2 expression in enteric serotonergic nerves in Hirschsprung’s-associated enterocolitis

Figure 5 Western blot analysis. Western blot analysis is seen in (A) showing reduced expression of TPH2 in the colon of children with HSCR complicated by HAEC, who were managed non-operatively prior to pull-through surgery. The reduction in expression is seen across the aganglionic and ganglionic bowel of these patients. Image (B) shows the impact of diverting stoma formation on TPH2 expression in children with HSCR and pre-operative HAEC, with increased expression seen when compared to children without a history of HAEC and no stoma. In both (A) and (B), protein expression has been normalized against the loading control, GAPDH (36kDa). TPH2: Tryptophan hydroxylase-2; HSCR: Hirschsprung’s disease; HAEC: Hirschsprung’s-associated enterocolitis.