Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. May 7, 2016; 22(17): 4354-4361
Published online May 7, 2016. doi: 10.3748/wjg.v22.i17.4354
Figure 3
Figure 3 The miR-125b mimic abrogats hepatitis C virus core protein-induced upregulation of cytokines. A: Real-time quantitative polymerase chain reaction analysis of miR-125b levels in THP-1 cells transfected with 0.4 nmol/L miRNA negative control (miR-control) or miR-125b mimic (miR-125b M), respectively; B: Twenty four hours after transfection, cells were treated with 5.0 μg/mL HCV core protein for an additional 6 h. The concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-10 and IL-6 in culture supernatants were analyzed by enzyme-linked immunosorbent assay. These data are representative of three experiments and shown as mean ± SEM (P < 0.001 by unpaired two-tailed Student’s t-test).