Mir-30d increases intracellular survival of Helicobacter pylori through inhibition of autophagy pathway

Figure 3 Mir-30d inhibitor upregulates autophagy in response to Helicobacter pylori infection in AGS and GES-1 cell lines. A: mir-30d expression in AGS and GES-1 cells with or without H. pylori infection at 48 h after mir-30d inhibitor transfection. ^aP < 0.05, with mir-30d inhibitor vs without mir-30d inhibitor; B: LC3B-I and LC3B-II protein levels in AGS and GES-1 cells with or without 24 h H. pylori infection; C: GFP-LC3 puncta in mir-30d transfected AGS and GES-1 cells with or without H. pylori infection (results shown as mean ± SD, ^aP < 0.05 vs control oligos transfected cells); D: Autophagosome and autophagolysosome in mir-30d transfected AGS and GES-1 cells with or without H. pylori infection (results shown as mean ± SD, ^aP < 0.05 vs control oligos transfected cells). H. pylori: Helicobacter pylori.