Mir-30d increases intracellular survival of Helicobacter pylori through inhibition of autophagy pathway

Figure 2 Mir-30d mimic represses autophagy in response to Helicobacter pylori infection in AGS and GES-1 cell lines. A: mir-30d expression in AGS and GES-1 cells with or without H. pylori infection at 48 h after mir-30d mimic transfection. Results shown as mean ± SD, ^aP < 0.05 vs control; B: The protein levels of LC3B-I and LC3B-II in mir-30d mimic transfected AGS and GES-1 cells with or without 24 h H. pylori infection; C: GFP-LC3 puncta in mir-30d transfected AGS and GES-1 cells with or without H. pylori infection (Results shown as mean ± SD, ^aP < 0.05 vs control mimic transfected cells); D: Quantification of autophagosome and autophagolysosome in mir-30d mimic transfected AGS and GES-1 cells with or without 24 h H. pylori infection (results shown as mean ± SD, ^aP < 0.05 vs control mimic transfected cells). H. pylori: Helicobacter pylori.