Combined detection of plasma GATA5 and SFRP2 methylation is a valid noninvasive biomarker for colorectal cancer and adenomas

Table 2 Clinicopathological features and DNA hypermethylation in plasma samples of 57 colorectal cancer patients and 30 patients with adenomas

Parameters	n	GATA5			SFRP2			ITGA4
		M	U	P value	M	U	P value	M	U	P value
Colorectal cancer
Gender
Male	34	20	14	0.627	21	13	0.174	10	24	0.157
Female	23	15	8		10	13		11	12
Age, yr
≤ 60	28	16	12	0.516	16	12	0.681	8	20	0.203
> 60	29	19	10		15	14		13	16
Tumor size, cm
< 5	42	22	20	0.019	21	21	0.266	13	29	0.123
≥ 5	15	13	2		10	5		8	7
Differentiation
Well	6	2	4	0.038	2	4	0.012	0	6	0.007
Moderately	36	20	16		16	20		11	25
Poorly	15	13	2		13	2		10	5
TNM stage
I-II	33	15	18	0.008	14	19	0.034	8	25	0.021
III-IV	24	20	4		17	7		13	11
Lymph node metastasis
N0	33	15	18	0.008	14	19	0.034	8	25	0.021
N1–3	24	20	4		17	7		13	11
Distant metastasis
M0	53	31	22	0.151	27	26	0.168	19	34	0.620
M1	4	4	0		4	0		2	2
Location
Colon	19	10	9	0.336	12	7	0.347	6	13	0.560
Rectum	38	25	13		19	19		15	23
Adenomas
Tumor size, cm
≤ 1	14	5	9	0.431	5	9	0.201	4	10	1.000
> 1	16	8	8		7	9		5	11
Tumor number
1	26	13	13	0.113	12	14	0.13	8	18	1.000
≥ 2	4	0	4		0	4		1	3
Intraepithelial neoplasia
Low	24	11	13	0.673	8	16	0.184	6	18	0.329
High	6	2	4		4	2		3	3