Screening analysis of candidate gene mutations in a kindred with polycystic liver disease

Table 1 Primers for amplification of PKD1 b long-range polymerase chain reaction

Fragment	Size (bp)	Exon	Forward primer	Reverse primer	Temperature(°C)
Exon 1	2278	1	5’-CGCAGCCTTACCATCCACCT-3’	5’-TCATCGCCCCTTCCTAAGCA-3’	64
Exons 2-7	4041	2-7	5’-CCCCGAGTAGCTGGAACTACAGTTACACACT-3’	5’-CGTCCTGCTGTGCCAGAGGCG-3’	70
Exons 8-12	3893	8-12	5’-ACGTCTGCGAGCTGCAGCCC-3’	5’-CTGCAGGGACAGGCGTCAGTGA-3’	70
Exons 13-15	4391	13-15	5’-TGGAGGGAGGGACGCCAATC-3’	5’-GTCAACGTGGGCCTCCAAGT-3’	65
Exons 15-21	5253	15-21	5’-ATCCCTGGGGGTCCTACCATCTCTTA-3’	5’-ACACAGGACAGAACGGCTGAGGCTA-3’	68
Exons 22-26	3276	22-26	5’-ATGCTTAGTGAGGAGGCTGTGGGGGTCCA-3’	5’-GCTTAAAGGGGAATGGCTTAAACCCG-3’	70
Exons 27-34	3916	27-34	5’-CGGGTCACCGGTTGTGGCA-3’	5’-ATGAGGCTCTTTCCACAGACAACAGAGGTT-3’	70
Exons 35-41	2632	35-41	5’-CAAGAGGCTCAAGAAACTGCCCG-3’	5’-GGGCTGTGGAAGCCGCCTA-3’	68
Exons 42-46	2370	42-46	5’-GAGTAGTTCTCCAGGAGTGCCG-3’	5’-ATTCTGCCTGGCCCTCGGCCTT-3’	63