In vitro identification of nonalcoholic fatty liver disease-related protein hnRNPM

Figure 5 Effects of glyceraldehyde modification of heterogeneous nuclear ribonucleoprotein M. Hep3B cells were incubated for 5 d and incubated with or without 4 mmol/L glyceraldehyde for 6 h. Cell lysates were separated on two-dimensional gradient sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) (A, B); or SDS-PAGE and probed with anti-Glycer-AGEs antibody (C). A: Control sample; B: Glyceraldehyde sample. The arrow shows spot identified with heterogeneous nuclear ribonucleoprotein M (hnRNPM).