Electroacupuncture activates enteric glial cells and protects the gut barrier in hemorrhaged rats

Figure 7 Intestinal zona occludens-1 protein expression at 6 h after blood loss. Intestinal extracts were obtained from rats at 6 h after blood loss to measure zona occludens (ZO)-1 protein expression using western blotting. Representative blots for ZO-1 protein are shown with corresponding GAPDH loading control to demonstrate equal protein load in all lanes. Electroacupuncture (EA) ST36 resulted in preservation of protein expression. Significant reduction in ZO-1 expression was seen in all the other groups. ^aP < 0.05 vs sham group; ^cP < 0.05 vs hemorrhagic shock (HS) group. (n = 3-6 rats per group at 6 h after blood loss). α-BGT: α-bungarotoxin; VGX: Vagotomy.