Dextran sulfate sodium-induced acute colitis impairs dermal lymphatic function in mice

Figure 2 Intravital fluorescent lymphangiography 30 min after oral gavage administration of Bodipy-FL-C16. Lymphangiography showing mesenteric lymphatic drainage in control (A, n = 4) and mice with dextran sulfate sodium (DSS)-induced colitis (B, n = 5) after oral gavage of 1 mL of Bodipy-FL-C16. Significantly reduced number of fluorescent mesenteric lymphatic vessels was observed in mice with DSS-induced colitis as compared to control mice (E). Scale bar = 2 mm. IHC assessment of lymphatic vessels using antibody against LYVE-1 in the colon of control (C) and mice with DSS-induced colitis (D) and quantification of number of lymphatic vessels (F) and vessel area (G) in colons. Data presented as mean ± SE. ^aP < 0.05 control vs DSS day 7.