Hepatitis B virus infection and genotype in asymptomatic people from 10 ethnic groups in Yunnan, China

Table 1 The primer sequences for identifying the hepatitis B virus genotypes/subgenotypes

Gene identification	PCR	Primer	Primer sequence	Site
Genotype	1st PCR	P1 (s)	5’-TCA CCA TAT TCT TGG GAA CAA GA-3’	nt2823–2845
(A-F)		S1-2 (as)	5’-CGA ACC ACT GAA CAA ATG GC -3’	nt685–704
	2nd PCR	B2 (s)	5’-GGC TCM AGT TCM GGA ACA GT-3’	nt67–86
	mixA	BA1R (as)	5’-CTC GCG GAG ATT GAC GAG ATG T-3’	nt113–134
		BB1R (as)	5’-CAG GTT GGT GAG TGA CTG GAG A-3’	nt324–345
		BC1R (as)	5’-GGT CCT AGG AAT CCT GAT GTT G-3’	nt 165–186
	mixB	BD1 (s)	5’-GCC AAC AAG GTA GGA GCT-3’	nt 2979–2996
		BE1 (s)	5’-CAC CAG AAA TCC AGA TTG GGA CCA-3’	nt 2955–2978
		BF1 (s)	5’-GYT ACG GTC CAG GGT TAC CA-3’	nt 3032–3051
		B2R (s)	5’-GGA GGC GGA TYT GCT GGC AA-3’	nt 3078–3097
Subgenotype	1st PCR	PC1 (s)	5’-CAT GCA ACT TTT TCA CCT CTG CCT-3’	nt1813-1836
B (B1 and B2)	2nd PCR	PC2 (s)	5’-ATT AGA CCT ATT GAT TGG AAA GT-3’	nt1861-1881
		COR-HBV (as)	5’-GAG TGC GAA TCC ACA CTC CA-3’	nt2285-2266
C (C1 and C2)	1st PCR	HBV964F (s)	5’-ATT AGA CCT ATT GAT TGG AAA GT-3’	nt964-986
	2nd PCR	HBV970F2 (s)	5’-CCT ATT GAT TGG AAA GTA TGT CA-3’	nt970-992
		HBV1272R (as)	5’-AGT ATG GAT CGG CAG AGG AG-3’	nt1272-1253

(s): Sense; (as): Anti-sense; M: A or C; Y: C or T; HBV: Hepatitis B virus; PCR: Polymerase chain reaction.