Sulforaphane-rich broccoli sprout extract improves hepatic abnormalities in male subjects

Figure 4 Effects of intake of broccoli sprout extract on oxidative stress and enzyme activity of glutathione S-transferase in livers from N-nitrosodimethylamine-induced chronic liver failure model rats. A: Thiobarbituric acid reactive substances (TBARS) levels were determined in livers from serum activities of alanine aminotransferase (AST) and alanine aminotransferase (ALT) in male Sprague-Dawley rats who had received an AIN-76 diet containing no (sham, n = 6 and control, n = 8) or different amount of BS extract at glucoraphanin (GR) doses of 62.5, 125, and 250 mg per 100 g diet (BS-low, BS-middle, and BS-high, respectively, n = 8) for 4 wk. All rats, except for sham group, were intraperitoneally injected with NDMA (5 mg per kg body weight) in 3 consecutive days of the week for 4 wk; B: GST activities in rat livers were determined with 1-chloro-2,4-dinitrobenzene (CDNB) as substrate. Data are shown as mean ± SE. ^aP < 0.05, ^bP < 0.01 vs sham, ^dP < 0.01, ^fP < 0.001 vs control (A: Tukey Kramer; B: Dunnett test).