Rapid and quantitative detection of hepatitis B virus

Table 1 Characteristics of isothermal amplification methods and polymerase chain reaction reviewed in this article

Method	Temperature requirement (°C)	No. of enzymes	Primer design	Product detection method	Rapid detection possibility	Tolerance to biological components
PCR	55-95	1	Simple	GE, ELISA, Real-time	Yes	No
IAM
LAMP	60-65	1	Complex	GE, turbidity, Real-time	Yes	Yes
TMA	50-60	2	Simple	GE, ELISA, Real-time, ECL	Yes	No
NASBA	37-41	2 or 3	Simple	GE, ELISA, Real-time, ECL	Yes	No
RCA	37	1	Simple	GE, Real-time	Yes	No

ECL: Electrochemiluminescence; GE: Gel electrophoresis; PCR: Polymerase chain reaction; IAM: Isothermal amplification methods; LAMP: Loop-mediated isothermal amplification; TMA: Transcription-mediated amplification; NASBA: Nucleic acid sequence-based amplification; RCA: Rolling circle amplification.