β-escin reverses multidrug resistance through inhibition of the GSK3β/β-catenin pathway in cholangiocarcinoma

Figure 2 Expression of P-gp down-regulated by β-escin. A: Expression of MRP mRNA analyzed using real-time PCR after treatment with β-escin for 24 h; B: Expression of MDR mRNA analyzed using real-time PCR (upper panel) and agarose gel electrophoresis analysis (lower panel) after treatment with β-escin for 24 h. Data were normalized to GAPDH; C: Expression of P-gp protein detected by ICC. Zoom: × 400; D: Expression of P-gp protein detected by western blot. β-Actin used as loading control. E: β-escin. ^aP < 0.05, ^bP < 0.01 vs control. PCR: Polymerase chain reaction; MDR: Multidrug resistance; MRP: MDR-related protein; P-gp: P-glycoprotein; ICC: Immunocytochemistry.