Upregulation of nemo-like kinase is an independent prognostic factor in colorectal cancer

Figure 5 Knockdown of nemo-like kinase expression affects viability, colony formation, and migration of colorectal cancer cells. A: Nemo-like kinase (NLK) mRNA expression was assessed in six CRC cell lines using quantitative real-time-PCR; Knockdown of NLK B: mRNA and C: Protein expression in HT-29 cells 48 h after infection with a lentivirus carrying NLK (Lv-shNLK) or control (Lv-shCon) shRNA; D: Cell viability MTT assay; E: Colony formation assay at 14 d after infection; F: Transwell tumor cell migration assay. ^bP < 0.01, ^cP < 0.05; ^dP < 0.01; ^fP < 0.01.