Review
Copyright ©The Author(s) 2015.
World J Gastroenterol. Jul 28, 2015; 21(28): 8527-8540
Published online Jul 28, 2015. doi: 10.3748/wjg.v21.i28.8527
Table 1 Circulating RNA specifically deregulated in pancreatic cancer
Target candidateUp/downSampleNumber of patientsExtraction methodQuantification methodTarget selectionNormalizationPotential valueRef.
mRNA
CEA mRNAUpWhole blood9 PK, 9 HCAGPCRT-PCRPreACTBD[16]
EGFR mRNAUpSerum11 PK, 23 HCN/ART-PCRPreB2MD[33]
4GnT mRNAUpWhole blood55 PK, 10 CP, 70 HCRNeasy Mini kitRT-PCRPreGAPDHD[34]
COL6A3 mRNAUpSerum44 PK, 46 BT, 30 HCPureYield RNA MidiprepqRT-PCRPreGAPDHD/P[35]
miRNA
mIR-155UpPlasma49 PK, 36 HCTRIzol LSTaqmanPremiR-16D[55]
miR-196aUpD
miR-21UpD
miR-210UpD
miR-155UpPancreatic juice16 PK, 5CPmirVana PARIS kitTaqmanPremiR-199 U6-snRNAD[68]
miR-21UpD
miR-196aUpSerum35 PK, 15 CP, 15 HCTRI Reagent BDTaqmanPrecel-miR-39D/P[69]
miR-200aUpSerum45 PK, 11 CP, 32 HCmirVana miR isolation kitTaqmanPremiR-16D[70]
miR-200bUpD
miR-210UpPlasma11 PK, 14 HCBoilingTaqmanPrecel-miR-54D[71]
miR-18aUpPlasma36 PK, 30 HCmirVana PARIS kitTaqmanPresynthetic reference panelD/T[72]
miR-16UpPlasma140 PK, 111 CP, 68 HCTRI Reagent BDTaqmanPrecel-miR-39D[73]
miR-196aUpD
miR-185UpSerum80 PK, 129 HCTRIzol LSTaqmanSequenceserum volumeD[74]
miR-191UpD
miR-20aUpD
miR-21UpD/P
miR-24UpD
miR-25UpD
miR-99aUpD
miR-1290UpSerum41 PK, 38 BT, 35 CP, 19 HCmirVana PARIS kitTaqmanTaqman mAmiR-16D[75]
miR-221UpPlasma47 PK, 30 HCmirVana PARIS kitTaqmanPresynthetic reference panelD/T/P[76]
miR-375DownD/T/P
miR-375UpPlasma48 PK, 47 HCTotal RNA purification kit (Norgen)TaqmanAffymetrix mAcel-miR-39,cel-miR-54, cel-miR-238D[77]
miR-27a3pUpWhole blood129 PK, 103 BT, 60HCTrizolTaqmanSequenceU6 snRNAD/S[78]
miR-196aUpSerum19 PK, 10 CP, 20 BT, 10 HCmiReasy RNA extraction kitTaqmanPremiR-24D[79]
miR-196bUpD
miR-205UpPancreatic juice50 PK, 19 CP, 19 HCTRIzol LSTaqmanAgilent mAU6 snRNAD/P[80]
miR-210UpD/P
miR-492UpD/P
miR-1427UpD/P
miR-22UpPlasma11 PK, 11 HR, 11 HCTRI Reagent BDTaqmancustom mAmiR-3196D[81]
miR-642bUpD
miR-885-5pUpD
Multi gene indexWhole blood409 PK, 25 CP, 312 HCPAXgene blood RNATaqmanTaqman mAath-miR159aD[82]
miR-483-3pUpPlasma32 PK, 12 BT, 30 HCmirVana PARIS kitTaqmanPremiR-16D[83]
miR-21UpD
snRNA
U2 snRNAUpSerum80 PK, 129 HCmirVana miR isolation kitqRT-PCRAgilent mAcel-miR-54D[84]