Neuron-glial antigen 2 overexpression in hepatocellular carcinoma predicts poor prognosis

Table 2 Immunohistochemical scoring system

	Score
Percent positive
0%	0
1%-10%	1
11%-50%	2
51%-80%	3
> 80%	4
Staining intensity
No staining	0
Weakly stained	1
Moderately stained	2
Strongly stained	3

The neuron-glial antigen 2 (NG2) expression score was calculated by multiplying the percent positive score by the staining intensity score; these scores ranged from 0-12. The tumors were divided into the following groups: negative (-, score of 0), low expression (1+, scores 1-4), moderate expression (2+, scores 5-8), and strong expression (3+, scores 9-12). The NG2 immunohistochemistry results were used to divide the samples into two groups: low expression (0 and 1+) and high expression (2+ and 3+).