E2F-1 overexpression inhibits human gastric cancer MGC-803 cell growth in vivo

Figure 7 Overexpression of E2F-1 alters protein expression of apoptosis regulators. A: Western blot analysis of c-Myc, S-phase kinase-associated protein 2 (Skp2), Bcl-2, cyclin D1, survivin, Bax and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; internal control) in the MGC-803 tumor tissues. Lanes: 1, LV-GFP-E2F-1 group; 2, LV-GFP-NC group; 3, phosphate-buffered saline (PBS) group; B: Quantification showing downregulation c-Myc, Skp2, Bcl-2, cyclin D1, survivin and upregulation of Bax protein levels (n = 8 animals for each condition). ^aP < 0.05 using analysis of variance and Student-Newman-Keuls analysis.