E2F-1 overexpression inhibits human gastric cancer MGC-803 cell growth in vivo

Figure 6 Overexpression of E2F-1 alters mRNA expression of apoptosis regulators. A: Agarose gel analysis of semiquantitative real-time polymerase chain reactions for c-Myc, S-phase kinase-associated protein 2 (Skp2), Bcl-2, cyclin D1, survivin, Bax and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) in the MGC-803 tumor tissues. Lanes: 1, LV-GFP-E2F-1 group; 2, LV-GFP-NC group; 3, phosphate-buffered saline (PBS) group; M, 500 bp marker; B: Quantification showing downregulation of c-Myc, Skp2, Bcl-2, cyclin D1, survivin and upregulation of Bax mRNA levels (n = 8 animals for each condition). ^aP < 0.05 using analysis of variance and Student-Newman-Keuls analyses.