Copyright
©The Author(s) 2015.
World J Gastroenterol. Jan 14, 2015; 21(2): 491-501
Published online Jan 14, 2015. doi: 10.3748/wjg.v21.i2.491
Published online Jan 14, 2015. doi: 10.3748/wjg.v21.i2.491
Table 1 Sequences of primers used for semi-quantitative real-time polymerase chain reaction
| Gene | Primer | Base sequence | PCR product (bp) |
| E2F-1 | Forward | 5'-CCCAACTCCCTCTACCCT-3' | 217 |
| Reverse | 5'-CTCCCATCTCATATCCATCCTG-3' | ||
| Survivin | Forward | 5'-AAATGCACTCCAGCCTCTGT-3' | 311 |
| Reverse | 5'-TGTCGAGGAAGCTTTCAGGT-3' | ||
| Bax | Forward | 5'-CCAAGAAGCTGAGCGAGTGT-3' | 269 |
| Reverse | 5'-CCGGAGGAAGTCCAATGTC-3' | ||
| Bcl-2 | Forward | 5'-GACTTCGCCGAGATGTCCAG-3' | 259 |
| Reverse | 5'-CATCCCAGCCTCCGTTATCC-3' | ||
| Cyclin D1 | Forward | 5'-CCCTCGGTGTCCTACTTCAA-3' | 237 |
| Reverse | 5'-GGGGATGGTCTCCTTCATCT-3' | ||
| Skp2 | Forward | 5'-GCTGCTAAAGGTCTCTGGTGT-3' | 291 |
| Reverse | 5'-AGGCTTAGATTCTGCAACTTG-3' | ||
| c-Myc | Forward | 5'-TTCTCTCCGTCCTCGGATTC-3' | 282 |
| Reverse | 5'-GTAGTTGTGCTGATGTGTGG-3' | ||
| GAPDH | Forward | 5'-ACCACAGTCCATGCCATCAC-3' | 450 |
| Reverse | 5'-TCACCACCCTGTTGCTGTA-3' |
-
Citation: Wei WY, Yan LH, Wang XT, Li L, Cao WL, Zhang XS, Zhan ZX, Yu H, Xie YB, Xiao Q. E2F-1 overexpression inhibits human gastric cancer MGC-803 cell growth
in vivo . World J Gastroenterol 2015; 21(2): 491-501 - URL: https://www.wjgnet.com/1007-9327/full/v21/i2/491.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i2.491