MiR-19a promotes epithelial-mesenchymal transition through PI3K/AKT pathway in gastric cancer

Figure 3 MiR-19a induces an epithelial-mesenchymal transition phenotype. A: MiR-19a induces an epithelial-mesenchymal transition (EMT) phenotype in SGC7901 and NUGC-3 cells; B: Epithelial and mesenchymal markers ZEB1, ZEB2, Slug, vimentin, and E-cadherin are measured by qRT-PCR in miR-19a mimic or negative control (NC)-transfected SGC7901 and NUGC-3 cells. The results are shown as fold change compared with negative control. Data are the means ± SD of three independent experiments; C: The E-cadherin and vimentin protein levels are measured using Western blot analysis. β-actin protein serve as an internal (loading) control.