Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice

Figure 2 Hydrogen-rich water decreases the oxidative stress and nitrotyrosine formation in the liver after acetaminophen administration. Three days after a sub-lethal dose of 500 mg/kg acetaminophen (APAP) challenge, blood samples and liver tissues were harvested to evaluate the oxidative stress, inflammation and nitrotyrosine formation. A: Hydrogen-rich water (HRW) protected against APAP-induced elevated malonyldialdehyde (MDA) and myeloperoxidase (MPO) levels, decreased superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione peroxidase (GSH-px) activities; B: HRW inhibited the expression of 4-HNE in the liver; C: HRW inhibited the nitrotyrosine (NT) protein adduct formation in the liver (n = 6, mean ± SD, ^aP < 0.05 vs NS control group; ^bP < 0.05 vs HRW control group; and ^cP < 0.05 vs APAP + NS group).