Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery

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World Journal of Gastroenterology

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1007-9327

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Basic Study

World J Gastroenterol. Apr 14, 2015; 21(14): 4150-4158
Published online Apr 14, 2015. doi: 10.3748/wjg.v21.i14.4150

Table 1 List of primer sequences and Universal ProbeLibrary combinations used in this study

Primer	Sequence 5’ to 3’	UPL Probe
RPL32 Forward	AACTGGCCATCAGGGTCAC	#64
RPL32 Reverse	CACAACTGGAACTCCTGTCTATTC	#64
TNF Forward	TTGTCGCTACATCGCTGAAC	#32
TNF Reverse	CCAGTAGGGCGGTTACAGAC	#32
IL1β Forward	CCAATTCAGGGACCCTACC	#19
IL1β Reverse	CATGGCTGCTTCAGAAACCT	#19
IL18 Forward	ACTTTACTTTGTAGCTGAAAACGATG	#85
IL18 Reverse	TTTAGGTTCAAGCTTGCCAAA	#85
IL12 Forward	GAGGGTGAGTGAGTGCCTTG	#62
IL12 Reverse	ACTCCGCCTAGGTTCGACTT	#62
IL8 Forward	TTCTTCTTTATCCCCAAACTGG	#41
IL8 Reverse	CCACATGTCCTCAAGGTAGGA	#41
IL6 Forward	TGAACTCCCTCTCCACAAGC	#7
IL6 Reverse	GGCAGTAGCCATCACCAGA	#7
IL10 Forward	TCCAGTTTTACCTGGAAGACG	#8
IL10 Reverse	CCTTGATATCCTCCCCATCA	#8

Citation: Lapthorne S, Bines JE, Fouhy F, Dellios NL, Wilson G, Thomas SL, Scurr M, Stanton C, Cotter PD, Pereira-Fantini PM. Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery. World J Gastroenterol 2015; 21(14): 4150-4158
URL: https://www.wjgnet.com/1007-9327/full/v21/i14/4150.htm
DOI: https://dx.doi.org/10.3748/wjg.v21.i14.4150

Lapthorne S, Bines JE, Fouhy F, Dellios NL, Wilson G, Thomas SL, Scurr M, Stanton C, Cotter PD, Pereira-Fantini PM. Changes in the colon microbiota and intestinal cytokine gene expression following minimal intestinal surgery. World J Gastroenterol 2015; 21(14): 4150-4158 [PMID: 25892864 DOI: 10.3748/wjg.v21.i14.4150]

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