Fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes

Figure 4 FUT1 knockdown in Panc1-RG cells does not alter gemcitabine resistance. A: Expression of fucosyltransferase (FUT)1 mRNA in Panc1-RG cells stably expressing small hairpin (sh)RNA; B: Aspergillus oryzae lectin (AOL) blot of shNC- or shFUT1-expressing Panc1-RG cells; C: Flow cytometric analysis using Ulex europaeus agglutinin (UEA)-I; D: WST assay results of transfectant cells treated with various gemcitabine concentrations. Results are expressed as mean ± SD; ^aP < 0.05 vs shNC. CBB: Coomassie brilliant blue; NC: Negative control.