High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma

Figure 1 Representative immunohistochemistry in intrahepatic cholangiocarcinoma. A: Negative staining for high-mobility group box 1; B: Strong staining for high-mobility group box 1 was mainly localized in the nucleus, and effused to cytoplasm and extra milieu in inflamed or necrotic areas; C: Negative staining for vascular endothelial growth factor C; D: Strong staining for vascular endothelial growth factor C in cytoplasm; E: Representative staining for D2-40 in lymphatic endothelial cells. A cancer embolus in lymph-vessel is shown; magnification, × 200.