Heme oxygenase-1 protects rat liver against warm ischemia/reperfusion injury via TLR2/TLR4-triggered signaling pathways

Figure 4 Heme oxygenase-1 changes activation of Toll-interleukin-1R- containing adaptor inducing interferon-β and myeloid differentiation factor 88 dependent pathways. A: Whole cell lysates were prepared, immunoprecipitated with anti-Toll-interleukin (IL)-1R-containing adaptor inducing interferon-β (TRIF), and immune complexes were analyzed by immunoblotting with the indicated antibodies. Shown are data of representative (n = 4) experiments; B: Expression levels of heme oxygenase (HO)-1, Toll-like receptor (TLR)-2, TLR3 and TLR4 were analyzed in whole cell lysates by immunoblotting with the respective Abs. n = 4-5 per group; C: The cell lysates were subjected to immunoprecipitation with anti-myeloid differentiation factor 88 (MyD88), and then the immunoprecipitates were analyzed by SDS-PAGE and immunoblotted with the indicated antibodies; n = 4 per group. TBK: TANK binding kinase; IRAK: IL-1R-associated kinase; TRAF: Tumor necrosis factor receptor-associated factor; I/R: Ischemia/ reperfusion; CoPP: Cobalt protoporphyrin; ZnPP: Zinc protoporphyrin.