PBX3 promotes migration and invasion of colorectal cancer cells via activation of MAPK/ERK signaling pathway

Figure 4 Effect of pre-B-cell leukemia homeobox 3 overexpression on cell invasion is mediated by MAPK/ERK pathway. A: Expression levels of phospho-ERK1/2 were upregulated after overexpression and downregulated after silencing PBX3; B: Western blot showed that upregulation of phospho-ERK1/2 in PBX3-overexpressing cells was reduced after treatment with U0126 for 48 h; C: Boyden chamber assay showed that migration and invasion were significantly decreased after U0126 treatment in PBX3-overexpressing HT-29 and SW480 cells. Bar: 50 μm. D: The migrating and invading cells through the Transwell membrane were counted and plotted. Data are presented as mean ± SD of three independent experiments. P-values are calculated using Student’s t test.