Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity

Figure 5 Proliferation and Wnt activation in adenoma of APC^min/+ mice and human familial adenomatous polyposis. A: β-catenin staining on adenoma of human familial adenomatous polyposis and in APC^min/+ mice; B: Expression of acyl-CoA synthetase 5 (ACSL5), Wnt2B and β-catenin and palmitoylation of Wnt2B in human sporadic adenocarcinoma; C: General view staining with HE; D-F: Immunohistochemical staining with ACSL5, β-catenin, Wnt2B; G: Palmitoylation of Wnt2B in isolated mitochondria of human tissue, immunoprecipitated with Wnt2B, after treatment with palmostatin B, hydroxylamine (hydrochloride), and BMCC. Wnt2B as reference; H: Densitometry calculation of (G) with ImageJ. ^aP < 0.05 vs control.