Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity

Figure 1 Expression of acyl-coA synthetase 5 and Wnt2B in mitochondrion, cytoplasm, and nucleus. A: Western blot of mitochondrial and cytoplasmatic CaCo₂ cell fractions, fraction specific reference antibodies were β-actin (cytoplasm) and CoxIV (mitochondria); B: Wnt2B mRNA expression in the presence or absence of acyl-CoA synthetase 5 (ACSL5) was analyzed by realtime PCR and normalized for the reference gene cyclophilin. ^aP < 0.05 vs control.