Connexin 32 and 43 promoter methylation in Helicobacter pylori-associated gastric tumorigenesis

Table 2 Cx32 and Cx43 primer sequences, amplified fragment size and annealing temperature

Method	Gene			Primer sequence (5’→3’)	Amplified fragment size (bp)	Annealing temperature (°C)
Real-time RT-PCR	Cx32		F:	ATGAACTGGACAGGTTTGTAC	302	56
			R:	ATGTGTTGCTGGTGCAGCCA
	Cx43		F:	TGCAGCAGTCTGCCTTTCGTTG	219	56
			R:	CCATCAGTTTGGGCAACCTTG
	β-actin		F:	TGGACTTCGCAGCACAGCAGATGG	289	56
			R:	ATCTCCTTCTGCATCCTGTCG
MSP	Cx32	M	F:	GGGGCGGGTGCGGCGAT	245	64
			R:	CTCCGCGCCTACGTCCC
		U	F:	GGGGTGGGTGTGGTGAT	245	64
			R:	CTCCACACCTACATCCCAA
	Cx43	M	F:	AAATTGTAATATTTGGGTTTCAGCGC	156	58
			R:	AATAACGCCATCTCTACTCACCG
		U	F:	TTTTAAAATTGTAATATTTGGGTTTCAGTGT	161	56
			R:	AATAACACCATCTCTACTCACCACA
BSP	Cx32		F:	GGTTATTTTTTTGGTGGGGTTATG	313	58
			R:	ACCCAAACAAATCCCCTATAATCTC
	Cx43		F:	TGTTTTTTAAAATTGTAATATTTGGGTTTA	377	56
			R:	AAAAACAAACTCATCTAACCTTCCTATTC
MassArray	Cx32		F:	CAGTTT CAGCAGTTTTTGGGTTTTTTGG	484	60
			R:	TAACTCCCTATCCCCTAACTCCTTA
	Cx43		F:	ATGTTTTTGCAGGTTGGATCAGGAAAT	447	60
			R:	ACCAACAAATAAAAACAAAATTATTCC