Phage display creates innovative applications to combat hepatitis B virus

Table 2 Epitope mapping of anti-hepatitis B virus antibodies

Antibodies against	Phage library used	Epitopes	Remarks	Ref.
PreS1 region
MA 18/7	M13 pIII 6-mer	ALDPAY, SLDPGF, PDPGFN, QLDPGF	The dipeptide DP was conserved from rounds 2 to 4 among all the analyzed phagotopes. Selected peptides were mapped to amino acids 19-23 of the PreS1 region	Germaschewski et al[134]
MA 18/7	M13 15-mer liner	SDTRGDPVFNLPFQ APVDSVFDRAFSAYL	Isolated peptides contain motif similar to LDPAF	D'Mello et al[135]
MA BX-182	M13 pIII 12-mer linear	SVPPPHTRSASG	Peptide matched amino acids 17SVPNP21 of the PreS1	Zhang et al[144]
	M13 pIII 7-mer disulfide constrained	CTNPVLRSC	Peptide spatially matched some amino acids within the same region but in a C- to N-terminal direction
PreS2 region
HBsAg polyclonal sera	M13 pIII 12-mer linear	TANGFYRLPSGS	Peptide sequence mapped to amino acids 135-146 of the PreS2 region	Zhang et al[136]
S-region
MA RFHBs6	M13 pIII 15-mer linear	TSNTHAC(R/K)TCSNPSR	The peptide sequence is similar to amino acids 115-129 of the S region	Motti et al[140]
MA BA1	M13 pIII 15-mer linear	PHDGNRAFPRTKVTM HMPRDANRHHQHPST SSLGSDHNARWVKRF	Low sequence homology with the S region
MA 6H6B6	M13 pIII 12-mer linear	WPHNWWPHFKVK	Peptide sequence homology not found	Jolivet-Reynaud et al[137]
	M13 pIII 6-mer linear	QGFLPQ	Peptide sequence mapped to amino acids 101-105 of the S-region
MA H166, H5, H35, H53	M13 pIII 30-mer linear	1Peptide Xn-CXTC-Xn	Sequence motif of CXTC was mapped to amino acids 121-124 of the S-region	Chen et al[138]
IgG derived from S-HBsAg immunized chimpanzee	M13 pIII 6-mer linear	TRVPRR, SRLPLR, SRLPKR	Sequence motif SxxPxR was mapped to amino acids 117-122 of the S-HBsAg	Germaschewski et al[139]

¹x_n represents random flanking amino acid sequences. Position X is constituted either by arginine, lysine or valine. MA: Monoclonal antibody; HBsAg: Hepatitis B surface antigen; IgG: Immunoglobulin G.