Hepatitis C virus in the new era: Perspectives in epidemiology, prevention, diagnostics and predictors of response to therapy

doi:10.3748/wjg.v20.i29.9633

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 7, 2014; 20(29): 9633-9652
Published online Aug 7, 2014. doi: 10.3748/wjg.v20.i29.9633

Table 2 Qualitative and quantitative hepatitis C virus RNA assays available for clinical use

Assay	Manufacturer	Method	LOD, IU/mL(dynamic range, IU/mL)
Qualitative
Amplicor HCV v2.0	Roche molecular system	RT-PCR (manual)	50
Cobas Amplicor HCV v2.0	Roche molecular system	Semi-automated RT-PCR	50
Ampliscreen¹	Roche molecular system	Semi-automated RT-PCR	< 50
Versant HCV RNA	Siemens healthcare diagnostics	Semi-automated TMA	10
Procleix HIV-1/HCV¹	Chiron corporation	TMA (manual)	< 50
Quantitative
Amplicor HCV Monitor	Roche molecular system	RT-PCR (manual)	N/A (600-700000)
Cobas Amplicor HCV Monitor v2.0	Roche molecular system	Semi-automated RT-PCR	600 (600-500000)
Versant HCV 1.0 Assay K-PCR	Siemens healthcare diagnostics	Semi-automated RT-PCR	15 (15-100000000)
Abbott RealTime HCV Assay	Abbott diagnostics	Semi-automated RT-PCR	12 (12-100000000)
LCx HCV RNA Quantitative Assay	Abbott diagnostics	Semi-automated RT-PCR	25 (25-2630000)
Cobas Ampliprep/Taqman	Roche molecular system	Semi-automated RT-PCR	15 (15-100000000)

¹Used for blood screening only. HCV: Hepatitis C virus; HIV: Human immunodeficiency virus; RT-PCR: Reverse transcription-polymerase chain reaction; LOD: Limit of detection.

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Citation: Ansaldi F, Orsi A, Sticchi L, Bruzzone B, Icardi G. Hepatitis C virus in the new era: Perspectives in epidemiology, prevention, diagnostics and predictors of response to therapy. World J Gastroenterol 2014; 20(29): 9633-9652
URL: https://www.wjgnet.com/1007-9327/full/v20/i29/9633.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i29.9633

Ansaldi F, Orsi A, Sticchi L, Bruzzone B, Icardi G. Hepatitis C virus in the new era: Perspectives in epidemiology, prevention, diagnostics and predictors of response to therapy. World J Gastroenterol 2014; 20(29): 9633-9652 [PMID: 25110404 DOI: 10.3748/wjg.v20.i29.9633]

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