Screening of lymph nodes metastasis associated lncRNAs in colorectal cancer patients

Figure 3 Comparison between microarray data and quantitative real-time polymerase chain reaction results. AK021444, ENST00000425785, AK307796 and ENST00000465846 were determined to be differentially expressed by microarray in 3 colorectal cancer patients, and this result was validated by quantitative polymerase chain reaction result (qPCR). AK021444, ENST00000425785, and AK307796, 3 specifically up-regulated lncRNAs in metastatic lymph nodes (MLN), were confirmed to be highly expressed in MLN, while ENST00000465846 was confirmed to be down-regulated in MLN. The validation results for the four long noncoding RNAs (lncRNAs) indicated that the microarray data correlated well with the qPCR results. M: Metastatic lymph nodes; N: Normal lymph nodes; T: Tumor tissue of colorectal cancer.