Retrospective Study
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Jul 7, 2014; 20(25): 8179-8186
Published online Jul 7, 2014. doi: 10.3748/wjg.v20.i25.8179
Table 1 List of primers used in the study
DQB1 allelePrimersPCR productDRB1 allelePrimersPCR product
DQB1*05015’ 5’ CGGAGCGCGTGCGGGG 3’128DRB1*015’ TTGTGGCAGCTTAAGTTTGAAT 3’168
3’ 5’ GCTGTTCCAGTACTCGGCAA 3’5’ GCTGTTCCAGTACTCGGCAT 3’
DQB1*05025’ 5’ TGCGGGGTGTGACCAGAC 3’117DRB1*15/165’ TCCTGTGGCAGCCTAAGA G 3’310
3’ 5’ TGTTCCAGTACTCGGCGCT 3’5’ CGCTGCACTGTGAAGCTCTC 3’
DQB1*05035’ 5’ TGCGGGGTGTGACCAGAC 3’87DRB1*035’ GTTTCTTGGAGTACTCTAGGTC 3’222
3’ 5’ GCGGCGTCACCGCCCGA 3’5’ TGCAGTAGTTGTCCACCCG 3’
DQB1*06015’ 5’ GCCATGTGCTACTTCACCAAT 3’198DRB1*045’ GTTTCTTGGAGCAGGTTAAACA 3’262
3’ 5’ CACCGTGTCCAACTCCGCT 3’5’ CGCTGCACTGTGAAGCTCTC 3’
DQB1*06025’ 5’ CGTGCGTCTTGTGACCAGAT 3’121DRB1*115’ CACGTTTCTTGGAGTACTCTAC 3’179
3’ 5’ GCTGTTCCAGTACTCGGCAT 3’5’ CTGGCTGTTCCAGTACTCCT 3’
DQB1*06035’ 5’ GGAGCGCGTGCGTCTTGTA 3’127DRB1*125’ AGTACTCTACGGGTGAGTGTT 3’198
3’ 5’ GCTGTTCCAGTACTCGGCAT 3’5’ CTGTTCCAGGACTCGGCGA 3’
DQB1*06045’ 5’ CGTGTACCAGTTTAAGGGCA 3’254DRB1*13/145’ GTTTCTTGGAGTACTCTACGTC 3’234
3’ 5’ GCAGGATCCCGCGGTACC 3’5’ CGTAGTTGTGTCTGCA(GA)TAGG 3’
DQB1*02015’ 5’ GTGCGTATTGTGAGCAGAAG 3’205DRB1*075’ CCTGTGGCAGGG AAGTATA 3’232
3’ 5’ GCAAGGTCGTGCGGAGCT 3’5’ CCCGTAGTTGTGTCTGCACAC 3’
DQB1*0201/03025’ 5’ GACGGAGCGCGTGCGTCT 3’129DRB1*085’ AGTACTCTACGGGTGAGTGTT 3’227
3’ 5’ CTGTTCCAGTACTCGGCGG 3’5’ CCCGTATTGTGTCTGCAG 3’
DQB1*03015’ 5’ GACGGAGCGCGTGCGTTA 3’122DRB1*095’ GTTTCTTGAAGCAGGATAAGTTT 3’236
3’ 5’ AGTACTCGGCGTCAGGCG 3’5’ CCCGTAGTTGTGTCTGCACAC 3’
DQB1*0302/03035’ 5’ GACGGAGCGCGTGCGTCT 3’122DRB1*105’ CGGTTGCTGGAAAGACGCG 3’204
3’ 5’ AGTACTCGGCGTCAGGCG 3’5’ CTGCACTGTGAAGCT CTCAC 3’
DQB1*03035’ 5’ GACGGAGCGCGTGCGTCT 3’129DRB1-Exon25’ TTCGTGTCCCCACAGCACGTTTC 3’295
3’ 5’ CTGTTCCAGTACTCGGCGT 3’5’ GCCGCTGCACTGTGAAGCTCTC 3’
DQB1*04015’ 5’ CACCAACGGGACCGAGCT 3’200Internal control (HGH)5′ CAGTGCCTTCCCAACCATTCCCTTA 3′439
3’ 5’ GGTAGTTGTGTCTGCATACG 3’5’ ATCCACTCACGGATTTCTGTTGTGTTTC 3′
DQB1*04025’ 5’ CACCAACGGGACCGAGCG 3’200
3’ 5’ GGTAGTTGTGTCTGCATACG 3’